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The PARS-I and PARS-II domains control the stability of RTA. 293T cells were cotransfected with the RTA expression vector pCR3.1-ORF50 and its deletion mutants as shown in Fig. 5B, with or without a vSP-1 expression construct, for 48 h. RTA expression levels were examined by Western blotting using an anti-RTA antibody. Lane M, mock transfection; lanes E, empty vector. β-Actin was used as a control for equivalent sample loading.
