Table 1.

Oligonucleotide primers used in this study

Primer target or function	Sequence
T3.0	5′-GGAATTCCTCAGTCACGGAAGTAATTACGC-3′
	5′-CTGCTCGAGTACATGGCGCAAGATGACAAGGTAAAG-3′
T3.0 1st mut	5′-GAG GCG ACC CGA CAT CCC AAG TTT CAG GGC CCG CTT CGT CTA ACA G-3′
	5′-CTG TTA GAC GAA GCG GGC CCT GAA ACT TGG GAT GTC GGG TCG CCT C-3′
T3.0 2nd mut	5′-GCA CAC CTT GGT CTC CGT CAA GAC CGC CCG GAA GCT CTT CGC CC-3′
	5′-GGG CGA AGA GCT TCC GGG CGG TCT TGA CGG AGA CCA AGG TGT GC-3′
vSP-1	5′-CGGGATCCATGTTTCAGGGCCCGCTTCGTCTAACA-3′
	5′-GGAATTCCCAAGGTGTGCCGTGTAGAGA-3′
vSP-2	5′-GGAATTCATGACCGCCCGGAAGCTCTTCGC-3′
	5′-GGGCGAAGAGCTTCCGGGCGGTCTTGACGGAGACCAAGGTGTGC-3′
ORF50 (in the pCMV-cMYC-1 vector)	5′-GGAATTCATGGCGCAAGATGACAAGGGTAAGAAG-3′
	5′-CCGCTCGAGTCAGTCTCGGAAGTAATTACGCCATTG-3′
Flag tag insertion upstream of the vSP-1 AUG within the T3.0 transcript	5′-CGACCCGACATCCCATGGACTACAAGGATGACGACGATAAGATGTTTCAGGGCCC-3′
	5′-GGGCCCTGAAACATCTTATCGTCGTCATCCTTGTAGTCCATGGGATGTCGGGTCG-3′
Flag tag insertion upstream of the vSP-2 AUG within the T3.0 transcript	5′-CCTTGGTCTCCGTCATGGACTACAAGGATGACGACGATAAGATGACCGCCCGGAA-3′
	5′-TTCCGGGCGGTCATCTTATCGTCGTCATCCTTGTAGTCCATGACGGAGACCAAGG-3′
GAPDH	5′-TGATGACATCAAGAAGGTGGTGAAG-3′
	5′-TCCTTGGAGGCCATGTGGGCCAT-3′