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Binding of filovirus GP to immobilized heparin. Concentrated lentiviral pseudotypes bearing the different filovirus envelope glycoproteins, Lassa virus envelope glycoprotein, or no envelope were incubated with heparin-Sepharose beads. After several washes, bound virus was subsequently eluted with 2 M NaCl. Heparin-bound virus was detected by SDS-polyacrylamide gel electrophoresis and Western blotting for the p24 protein.
