Fig 2.

Soluble GAGs inhibit filovirus GP-mediated infection of 293T cells. (A) 293T cells were infected with concentrated HIV pseudotypes bearing the different filovirus envelope glycoproteins and Lassa virus envelope glycoprotein in the presence or absence of different concentrations of soluble heparin, chondroitin sulfate A, dermatan sulfate, or heparan sulfate. Results are expressed as relative light units (RLU) and represent the means ± SD for duplicate wells. The results are representative of at least three different experiments. Statistics consisted of on one-way analysis of variance (ANOVA) followed by Bonferroni posttests. *, P < 0.05; **, P < 0.01; ***, P < 0.001. (B) HIV pseudotypes were subjected to SDS–10% PAGE, transferred to nitrocellulose, and immunoblotted for the presence of the C-terminal V5 epitope using mouse anti-V5. Anti-p24 was used as a loading control.