Fig 3.

Replication of mutant MCMVs in cell culture. (A and B) 10.1 fibroblasts or RAW264.7 macrophages were infected with wt-l, m41.1ko, or rev virus at an MOI of 0.02. Supernatants were collected, and viral titers were determined by plaque assay or the TCID₅₀ method. (C and D) BMDMs were isolated as described previously (31). On day 7 after isolation, cells were infected with wt-l, m41.1ko, or rev virus at an MOI of 0.02. Supernatants were collected and viral titers were determined by plaque assay. All experiments were done in duplicate or triplicate; means and SEM are shown.