Fig 6.

TPV-15L knockout virus. (A) Confirmatory PCR after three rounds of plaque purification using primers internal to TPV-2L (2L inter) and TPV-15L (15L inter) ORFs. As indicated by arrows, the TPV-2L internal fragment is expected to be 903 bp, and the TPV-15L internal fragment is expected at 197 bp. (B and C) An isolated plaque of TPVΔ15LCherry in OMK cells at 5 days postinfection (dpi), expressing mCherry as visualized by fluorescence (B) and phase contrast thereof (C). (D and E) An isolated plaque of wtTPV in OMK cells visualized by fluorescence (D) and phase contrast (E). Isolated plaques were selected from a 6-well titration plate.