Table 1.
Primary antibodies used for immunofluorescence and immunoblotting
| Antigen | Antibody | Typea | Source | Dilution used for: |
|
|---|---|---|---|---|---|
| Immunofluorescenceb,d | Immunoblottingc,d | ||||
| EEA-1 | 14/EEA1 | M | BD Transduction | 1:250 | ND |
| UL44 | 10D8 | M | Virusys | ND | 1:2,500 |
| Golgi marker proteins | |||||
| giantin | Custom | M | Gifte (Abcam) | 1:250 | ND |
| GM130 | Clone 35 | M | BD Transduction | 1:100 | ND |
| EP892Y | R | Abcam | 1:250 | ND | |
| GS27 | 25 | M | BD Transduction | 1:1,100 | ND |
| β-Actin | ACTN05(C4) | R | Abcam | ND | 1:1000 |
| pp28 | 10B4-29 | M | Giftf | 1:20 | 1:50 |
| IE1/IE2 | 3H4 | M | Giftg | ND | 1:100 |
| gB | 27-156 | M | Gifth | 1:30 | ND |
| CD63 | SC-5275 | M | Santa Cruz | 1:250 | ND |
a
R, rabbit; M, mouse monoclonal.
b
Dilution in PBS supplemented with bovine serum albumin and Tween 20.
c
Dilution in TBS–5% milk supplemented with bovine serum albumin and Tween 20.
d
ND, not done.
e
Generous gift from Samuel Campos, University of Arizona.
f
Generous gift from Michael Nevels, University of Regensburg.
g
Generous gift from Tom Shenk, Princeton University.
h
Generous gift from Bill Britt, University of Alabama at Birmingham.