Fig 10.

Effects of prestimulation with IFN-β on MeV-mediated oncolysis and viral replication. (A) Cells were prestimulated with IFN-β (1,000 U/ml) for 20 h (white bars) or were left unstimulated (black bars), followed by infection with MeV-SCD at different MOIs. At 96 hpi, remnant tumor cell masses were determined by SRB assay. Values were calculated relative to those of untreated controls. Means and SEM from three independent experiments are shown. (B) Cells were prestimulated with IFN-β for 24 h before infection with MeV-SCD at an MOI of 0.03 (gray dotted lines) or were left unstimulated (black lines). At the indicated time points, supernatants (upper panel) and cell lysates (lower panel) were harvested. Titration was performed on Vero cells. Means and SD from three independent experiments are shown.