Fig 5.

Effect of lysosomotropic agents on McKbac and gK mutant virus entry. Cells (CHO-nectin-1) were pretreated with different concentrations of ammonium chloride (A and C) and monensin (B and D) and infected with McKbac and McKbac gK mutants (A and B) or VSV (C and D) for 1 h at 34°C in the presence of the inhibitor. One hour after infection, the inoculum was removed and the cells were incubated in the presence of each inhibitor at 37°C until the cells were processed. The percentages of infected cells were determined after staining with anti-ICP4 antibody and analyzed by flow cytometry. Six hours after VSV infection, the percentage of GFP-positive cells were measured by flow cytometry. CHO-PILRα cells were pretreated with different concentrations of ammonium chloride (E), monensin (F), and bafilomycin (G), infected with McKbac, and processed for flow cytometry as described above.