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Polarity of NiV entry. Polarized MDCK cells were infected with NiV (MOI of 10) from either the apical (A) or basal (B) filter chamber. At 48 h p.i., the cells were fixed with 4% PFA, and virus-positive cells were stained with a NiV-specific antiserum and AF 488-labeled secondary antibodies. Bars, 100 μm.
