Fig 5.

Permeability of NiV-infected polarized MDCK cell monolayers. MDCK cells were cultivated for 5 days on permeable filter supports and were infected from the apical side at an MOI of 0.01 (low-dose NiV) or an MOI of 10 (high-dose NiV). Horseradish peroxidase (HRP) was added to the apical filter chamber of uninfected (mock) or NiV-infected cells at 24, 48, and 72 h p.i. After 1 h, HRP flowthrough from the apical to the basal medium was quantified by measuring the HRP activity in the basal medium. Values were normalized to HRP activity in the basal medium of an empty filter without cells (equal to 1). Error bars indicate standard deviations (n ≥ 3). Mock is shown as the mean of the values obtained at 24, 48, and 72 h in five independent experiments. OD, optical density.