Fig 7.

NiV envelope protein transport in the presence of monensin. Nonpolarized MDCK cells grown on coverslips were cotransfected with M and either the NiV F or G gene or were infected with NiV (MOI of 0.01). At 2 h after transfection or at 1 h p.i., 20 μM monensin was added to the medium. Twenty-four hours later, the cells were fixed and permeabilized with methanol. The envelope proteins were stained by using M-, F-, or G-specific antibodies. The glycoproteins were then visualized with AF 488-labeled anti-rabbit IgG antibodies (green), and the M protein was detected with AF 568-conjugated anti-mouse IgG antibodies (red). (A) Colocalization of M and F proteins in the absence of monensin (control w/o monensin) and in the presence of monensin (M/F cotransfection + monensin and NiV infection + monensin). (B) Colocalization of M and G proteins in the absence of monensin (control w/o monensin) and in the presence of monensin (M/G cotransfection + monensin and NiV infection + monensin). The insets represent expansions of the merged images. Bars, 10 μm.