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Viral replication is variable in parental HT1080, dnMEK (HT92-6), and caMEK (HT84-4) cell lines. Cells were exposed to 1 PFU of HSV-1(F) or R2621 mutant virus per cell in serum-free medium. At 24 h after infection, the amounts of viral DNA were determined by real-time PCR using a TaqMan probe specific for UL30.
