Fig 5.

CD8⁺ T cells, NK cells, and myeloid DC populations are decreased in the dLNs and spleens of WNV-infected Cd22^−/− mice. Pooled popliteal dLNs (A, B, and C) and spleens (D and E) were harvested from naive mice or mice infected with 10³ PFU of WNV-TX at the indicated days p.i. Total cells were stained for the indicated leukocyte populations. Total cell numbers for various populations are shown per dLN 1 day postinfection (A, B, and C) or per spleen (D and E) at the indicated time points. Statistics was performed using Student's t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. n.s., not significant; n.d., not detected. Data show one representative of 3 independent experiments with three mice per group per time point (dLNs) or mice from three independent experiments where n > 9 per group per time point (spleen).