Fig 7.

Impaired recruitment of T cells and NK cells into infected dLNs in Cd22^−/− mice. Ly5.1⁺ total splenocytes from WT C57BL/6 mice were adoptively transferred by i.v. injection into Ly5.2⁺ WT or Cd22^−/− mice. The next day, individual footpads of recipient mice were left uninjected (naive), injected with 10 μl of a 1% FCS-containing HBSS vehicle (mock), or injected with 10³ PFU of WNV-TX (infected). Twenty-four hours p.i., individual dLNs were harvested and processed separately before staining for the following populations: CD3⁺ T cells (A), NK cells (B), total CD4⁺ T cells (C), total CD8⁺ T cells (D), CD11c^hi DCs (E), and B cells (F). Symbols represent individual dLNs from mice from three independent experiments. Statistics were performed using a Mann-Whitney U test of the median. *, P < 0.05; **, P < 0.01. n.s., not significant.