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. 2012 Oct 19;3(4):671–685. doi: 10.3390/genes3040671

Figure 3.

Figure 3

Abundance of developmentally regulated transcripts in endo-RNAi mutants. (A) gpd-3 normalized bli-1 transcript abundance at mid-L4 (69 hours post hatching at 15 °C) in the indicated mutants. (B) Relative bli-1 transcript levels in eri-1(mg366) compared to N2 and sid-1(qt9) at mid-L4, normalized to the three indicated housekeeping genes. (C) Relative expression of the indicated developmental stage-specific genes at the denoted stages in eri-1(mg366) and rrf-3(pk1426) mutants compared to N2. At 15 °C, L1 worms had their RNA extracted 22 hours post-hatching, L4 worms had their RNA extracted 69 hours post-hatching, young adult (“YA”) worms had their RNA extracted 80 hours post-hatching, and mature adults (“MA”) worms had their RNA extracted 100 hours post-hatching. Error bars indicate standard deviation. * indicates p < 0.05; ** indicates p < 0.01; no asterisks indicate statistical insignificance. p-values are calculated by t-test.