Fig 3.

Phylogenetic analysis of partial mcrA sequences derived from RW (rumen wheat), RC (rumen corn), CW (cecum wheat), and CC (cecum corn) clone libraries. Evolutionary analyses were conducted in MEGA 5. There were a total of 390 positions in the final data set. The evolutionary history was inferred using the neighbor-joining method. The tree was bootstrap resampled 1,000 times; only bootstrap values > 50 are shown. The scale bar equals an average of 0.5 nucleotide substitutions per 100 positions. The GenBank accession numbers for reference nucleotide sequences are given between vertical bars. Numbers in parentheses indicate the number of sequences grouped in the same OTU at 87% species cutoff. Methanopyrus kandleri was used as an outgroup to root the tree.