Table 2.
Sequence similarities and predicted functions of the deduced proteins from the A. terreus dehydrocurvularin biosynthetic locus
| Gene | Gene length (bp) | Protein length (aa) | No. of exons | Predicted function | Conserved domain(s) | Best BLASTP hit in:a |
|||
|---|---|---|---|---|---|---|---|---|---|
| GenBank |
A. terreus NIH2624 |
||||||||
| Accession no. (organism) | E value (% identity) | Accession no. | E value (% identity) | ||||||
| orf1 | 2,276 | 673 | 4 | Phosphorylase | COG0775, nucleoside phosphorylase; pfam13191, ATPase domain | EED52644 (Aspergillus flavus) | 2e−169 (45) | EAU38850 | 1e−145 (45) |
| AtcurS2 | 6,485 | 2,083 | 5 | Dehydrocurvularin synthase nrPKS | SAT-KS-AT-PT-ACP-TE | EDU47223 (Pyrenophora tritici-repentis) | 0.0 (78) | EAU38791 | 0.0 (35) |
| AtcurR | 2,251 | 728 | 2 | Dehydrocurvularin biosynthesis regulator | smart00066, GAL4-like Zn2Cys6 binuclear cluster; smart00906, fungal specific transcription factor | EED53048 (Aspergillus flavus) | 0.0 (71) | EAU35011 | 0.0 (71) |
| AtcurE | 1,924 | 571 | 3 | Dehydrocurvularin exporter | cd06174, major facilitator superfamily | EED53049 (Aspergillus flavus) | 0.0 (85) | EAU35010 | 0.0 (80) |
| AtcurS1 | 7,468 | 2,387 | 6 | Dehydrocurvularin synthase hrPKS | KS-AT-DH- KRS-ER- KRC-ACP | EDU47225 (Pyrenophora tritici-repentis) | 0.0 (76) | EAU32666 | 0.0 (33) |
| orf2 | 672 | 223 | 1 | Metal-dependent phosphohydrolase | smart00471, metal-dependent phosphohydrolase | EFY87661 (Metarhizium acridum) | 3e−59 (47) | EAU37684 | 2e−118 (77) |
| orf3 | 624 | 170 | 3 | Hypothetical protein | NFb | EAW17244 (Neosartorya fischeri) | 4e−06 (29) | EAU37685 | 3e−68 (73) |
| orf4 | 2,181 | 726 | 1 | Glycosyl hydrolase | pfam00933, glycosyl hydrolase family 3, N terminus; pfam01915, glycosyl hydrolase family 3, C terminus | BAE55411 (Aspergillus oryzae) | 0.0 (75) | EAU37686 | 0.0 (90) |
a
Best BLASTP hit of the putative protein in the A. terreus NIH2624 genome and in the rest of GenBank.
b
NF, not found.