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Purification of C-terminally His6-tagged hydrocaffeyl-CoA:caffeate CoA transferase CarA of A. woodii. E. coli pET21a-carA was cultured at 37°C in LB medium. CarA was purified by affinity chromatography on Ni2+-NTA. Samples were separated on SDS-PAGE; proteins were stained with Coomassie blue or the recombinant His6 tag was detected via Western blotting.
