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Heterologously produced and purified CarA catalyzes CoA transfer from hydrocaffeyl-CoA to caffeate. The assay mixture contained 1 ml 100 mM KPi buffer (pH 7.5) and 0.15 μg CarA. (A) 250 μM caffeate and 45 μM hydrocaffeyl-CoA were added as indicated. (B) No enzyme added. (C) No caffeate added. The formation of caffeyl-CoA was followed spectrophotometrically at 346 nm.
