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Substrate specificity of the hydrocaffeyl-CoA:caffeate CoA transferase CarA. (A) Structures of phenyl acrylates and other substrates used. (B) The assay mixture contained 1 ml 100 mM KPi buffer (pH 7.5), 0.15 μg CarA, and 45 μM hydrocaffeyl-CoA. The reaction was started with the addition of 250 μM substrate. Formation of the corresponding CoA thioester was followed spectrophotometrically; 100% corresponds to 151 U/mg.
