Fig 1.
Expression analysis of T. onnurineus NA1 hydrogenase gene clusters by RT-PCR on MM1-CO. RT-PCR analysis of each large subunit of mbh (TON_1593), mbx (TON_0489), frh (TON_1560), sulfI (TON_0534), mch (TON_1023), mfh2 (TON_1569), and mfh1 (TON_0276) hydrogenases was performed with samples grown on MM1-CO with 0.5 g liter−1 of yeast extract and YPS medium. cha, a chaperonin-encoding gene, was used as a control to normalize the expression level. The numbers under PCR bands represent band intensities relative to those on YPS medium.