Fig 3.

Expression levels of genes in a codh-mch-mnh3 gene cluster of the MC01 mutant on MM1-CO. (A) The mRNA quantity of three genes (TON_1018, TON_1023, and TON_1031) of the wild-type strain and the MC01 mutant was measured by RT-qPCR and indicated as fold difference from the value of the wild-type strain, which is taken as 1. Error bars indicate the standard deviations from three independent experiments. (B) Western blot analysis of proteins encoded by TON_1018 (67.7 kDa) and TON_1023 (61.7 kDa). M, molecular mass marker; WT, wild-type strain. (C) H₂ production activity of cell lysates of the wild-type strain and MC01 mutant. Error bars indicate the standard deviations from three independent experiments.