Fig 6.

Alginate and free uronic acids produced by P. aeruginosa with chromosomal integration of genes for AlgE and its variants. (A) The amount of alginate, relative to the amount of alginate produced by PDO300ΔalgE (mini-CTX::algE). (B) The amount of free uronic acids, given as a percentage ratio between the filtrate and supernatant. Experiments were conducted in triplicate, and the error bars represent the standard deviations of the mean values. 300+miniCTX indicates PDO300 carrying the integration-proficient mini-CTX plasmid; 300ΔE+miniCTX shows PDO300ΔalgE carrying the mini-CTX plasmid; 300ΔE+miniCTX:E depicts PDO300ΔalgE carrying the mini-CTX::algE plasmid. Variants of AlgE are indicated as first letter E indicating wild-type AlgE, followed by M for the membrane segment or T for the periplasmic turn, followed by a number indicating their respective position from the N terminus of AlgE; F stands for the FLAG epitope insertion.