Fig 7.

Effects of Fur on litR, luxR, smcR, and hapR transcriptional reporters. In all panels, V. fischeri cultures of ES114 (wild type), YLM111 (Δfur), JB19 (litR::erm), or ANS63 (Δfur litR::erm) were grown in aerobic shake flasks in SWTO medium supplemented with 43 μM FeSO₄. In panels A and C, for litR, smcR, and hapR transcriptional reporters, cells harboring pAS120, pAS123, and pAS128 were harvested at an OD₅₉₅ of ∼0.5. In panel B, for the luxR transcriptional reporter, cells harboring pJLB170 were harvested at an OD₅₉₅ of ∼1.0. Error bars indicate standard deviations (n = 4 [A and B] and n = 3 [C]). Asterisks in panels A and C indicate a significant difference of the indicated pairwise comparison (P < 0.005) by Student's t test, while the comparison labeled “ns” was not significant (P > 0.05). In panel B, lowercase letters shared between bars indicate no statistically significant difference (P > 0.2), whereas different letters indicate a significant difference (P < 0.001), based on a one-way analysis of variance and Tukey's honestly significant difference test. Data in each panel are representative of at least three independent experiments.