Table 1.
Strains and plasmids used in this study
| E. coli strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| SM10λpir | Host for maintenance and conjugation of pDS132 derivatives | Lab collection |
| Sakai | O157:H7; stx1+ stx2+ | Wei Zhang, Division of Biology, Illinois Institute of Technology |
| Sakai-Strr | Streptomycin-resistant mutant of Sakai | This study |
| SK-18 | Second-crossover derivative of Sakai::(pDS132::ΔtDNA5); ΔSp11; type I deletion mutant | This study |
| SK-11 | Second-crossover derivative of Sakai::(pDS132::ΔtDNA5); type II deletion mutant | This study |
| SK-17 | Second-crossover derivative of Sakai::(pDS132::ΔtDNA5); type III deletion mutant | This study |
| SKΔpchB | Sakai with pchB deletion | This study |
| Plasmids | ||
| pDS132 | Suicide vector; Camr; encodes levansucrase | 34 |
| pDS132::ΔtDNA5 | pDS132 carrying concatenated fragments flanking ileZ5-argO5 | This study |
| pDS132::ΔtDNA1 | pDS132 carrying concatenated fragments flanking ileZ1-argN1-argO1 | This study |
| pDS132::ΔtDNA3 | pDS132 carrying concatenated fragments flanking ileZ3-argO3 | This study |
| pKD4 | Template plasmid for aph gene; Kanr | 35 |
| pKM200 | Red recombinase expression plasmid; Camr | 36 |