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ID50 of the Burkholderia symbiont for symbiotic colonization. In the oral administration experiments, second-instar nymphs were fed with different numbers of Burkholderia symbiont (strain RPE75) cells, and the percentage of host individuals subsequently colonized was determined by diagnostic PCR. The dose at which 50% of the animals become infected by the third instar, indicated by the dotted lines, was determined using the equation probit (y) = 0.37 ln (x) + 3.38.
