Table 3.
Monosaccharide composition of R. sullae EPS as determined by GC-MS
| EPS and strain | Carbon source | Monosaccharide composition (%)a |
||||||
|---|---|---|---|---|---|---|---|---|
| Glc | Gal | Fuc | Man | GalAb | Rha | Rib | ||
| LMW EPS | ||||||||
| R. sullae A6 | Mannitol | 32 ± 1 | 30.5 ± 2.5 | 37 ± 4 | <1 | <1 | <1 | ND |
| Sucrose | 16.5 ± 3.5 | 32.5 ± 7.5 | 51 ± 11 | <1 | <1 | ND | ND | |
| Sorbitol | 60.5 ± 10.5 | 22.5 ± 5.5 | <1 | 8.5 ± 8.5 | <1 | 8.5 ± 0.5 | ND | |
| R. sullae RHF | Mannitol | 38 ± 5 | 30.5 ± 3.5 | 30.5 ± 2.5 | <1 | <1 | <1 | ND |
| Sucrose | 33.5 ± 16.5 | 29 ± 4 | 37.5 ± 12.5 | <1 | <1 | <1 | ND | |
| Sorbitol | 37 ± 12 | 34 ± 3 | 27.5 ± 9.5 | <1 | <1 | ND | ND | |
| HMW EPS | ||||||||
| R. sullae A6 | Mannitol | 45 ± 5 | 32.5 ± 7.5 | 10 ± 10 | ND | >10 | <1 | ND |
| Sucrose | 30 ± 10 | 36.5 ± 3.5 | 17 ± 16 | 3.5 ± 3.5 | >10 | <1 | ND | |
| Sorbitol | 41.5 ± 8.5 | 25 ± 8 | ND | 8.5 ± 8.5 | <10 | 17 ± 0 | ND | |
| R. sullae RHF | Mannitol | 28.5 ± 14.5 | 28 ± 0 | 14 ± 14 | 28.5 ± 28.5 | ND | ND | ND |
| Sucrose | 46 ± 21 | 28.5 ± 3.5 | 12.5 ± 12.5 | <1 | >10 | ND | ND | |
| Sorbitol | 33 ± 0 | 33 ± 0 | 33 ± 0 | ND | <1 | ND | ND | |
| Total EPS | ||||||||
| R. sullae A6 | Mannitol | 42.7 ± 6.5 | 33.4 ± 1.0 | 19.9 ± 13.5 | <1 | <10 | <1 | ND |
| Sucrose | 24.7 ± 6.7 | 34.9 ± 2.0 | 30.4 ± 17.0 | 2.3 ± 1.5 | <10 | <1 | ND | |
| Sorbitol | 54.4 ± 9.5 | 23.3 ± 1.2 | <1 | 8.5 ± 0 | <10 | 11.2 ± 4.2 | ND | |
| R. sullae RHF | Mannitol | 34.1 ± 4.7 | 29.5 ± 1.2 | 23.6 ± 8.2 | 12.3 ± 12.3 | <1 | <1 | ND |
| Sucrose | 39.5 ± 6.3 | 28.8 ± 0.3 | 25.5 ± 12.5 | <1 | <10 | <1 | ND | |
| Sorbitol | 35.5 ± 2.0 | 33.6 ± 0.5 | 29.5 ± 2.7 | <1 | <1 | ND | ND | |
a
Glc, glucose; Gal, galactose; Fuc, fucose; Man, mannose; GalA, galacturonic acid; Rha, rhamnose; Rib, ribose; ND, not detected. When glucose was used as a carbon source, the composition was quite variable. Values are expressed as means ± standard deviations where applicable.
b
GalA does not demonstrate a good response factor when derivatized with TMS.