Table 4.
Chemical shift data for glycosyl residues of LMW EPS produced by R. sullae RHF grown on mannitol as determined by two-dimensional NMR in D2O at 500 MHz with the use of a cryoprobea
| Designation | Glycosyl residue | Assignments (δ, ppm)b |
|||||
|---|---|---|---|---|---|---|---|
| H-1/C-1 | H-2/C-2 | H-3/C-3 | H-4/C-4 | H-5/C-5 | H-6/C-6 | ||
| A | →4αGal1→ | 5.41/99.1 | 3.84/68.1 | 4.13/66.5 | 3.92/71.8* | 3.72/62.5 | 3.82; 3.77/60.6 |
| →4αGal(6 Succ)1→ | 3.92; 3.79/64.8 | ||||||
| B | →3αFuc1→ | 5.33/96.7 | 4.85/70.8 | 4.12/71.0* | 4.04/68.5* | 4.38/67.2 | 1.22/14.5 |
| C | →3αFuc1→ | 5.30/98.1 | 5.02/69.9 | 4.23/76.5 | 4.20/69.1 | 4.43/66.7 | 1.07/14.7 |
| D | α(4.6 Pyr)Glc1→ | 5.09/92.1 | 3.54/72.1 | 3.71/79.8 | 3.39/75.9 | 3.64/69.5 | 3.91; 3.82/67.7 |
| E | →4βGalA1→ | 4.88/100.8 | 3.86/80.0 | 3.90/78.4 | 3.73/76.5* | 3.68/76.8 | 175.0 |
| F | →3αFuc(4 Ac)1→ | 4.57/103.7 | 3.31/72.9 | 3.69/76.2 | 3.78/71.5 | 4.06/71.0 | 1.27/19.7 |
| G | →3βGlc | 4.53/96.0 | 3.25/74.1 | 3.51/82.6 | 3.42/67.7 | 3.67/61.5* | 3.70; 3.64/60.6 |
| Succ | 2.08/20.0 | 173.0 | 183.0 | ||||
| Pyr | 1.37/24.8 | 100.1 | 176.0 | ||||
| Ac | 1.86/22.4 | 181.1 | |||||
a
Succ, succinate; Pyr, pyruvate; Ac, acetate. All other abbreviations are as defined in Table 3, footnote a.
b
*, assignments that could be exchanged with one other. Assignments indicated in italics were determined by COSY 1H/1H and HSQC 13C/1H.