Figure 1.

Cortical sampling position (A) and labeled cells (B–D). The animals were lightly fixed with paraformaldehyde, and the brains were removed, trimmed and flattened, and sliced at 250 μm tangential to the cortical surface. Slices were incubated in 4,6-diamidino-2-phenylindole solution to visualize neuronal nuclei. Layer III pyramidal cells were individually injected with Lucifer yellow and reacted for diaminobenzidine product (B–D). Examples of injected cells from the primary visual cortex (V1), the inferior temporal cortex (TE), and the prefrontal cortex (PFC) of a 3-month-old monkey are shown in (B), (C), and (D), respectively. Abbreviations: LS, lateral sulcus; STS, superior temporal sulcus. Scale Bars = 300 mm (A), 100 μm (B–D).