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. 2013 Jan 15;61(9):2120–2129. doi: 10.1021/jf3046076

Figure 5.

Figure 5

Effect of various concentrations of superoxide dismutase (SOD) on TNF-induced adhesion molecules expression and superoxide generation: (A) various concentrations of SOD (100 and 200 U/mL) and IRW and IQW (50 mol/L) on ICAM-1 expression; (B) various concentrations of SOD (100 and 200 U/mL) and IRW and IQW (50 mol/L) on VCAM-1 expression. Confluent HUVEC monolayers were pretreated with peptides for 20 h and then treated with SOD (100 and 200 U/ML) for 1 h prior to 4 h of incubation with 5 ng/mL TNF. ICAM-1 and VCAM-1 protein levels are expressed as fold increase over the untreated control. Bars represent mean values (mean ± SEM, n = 4 independent experiments). Representative Western blots are shown below. (∗∗∗) P < 0.001, (∗∗) P < 0.01, and (∗) P < 0.05, compared to TNF alone. (C) Effect of various concentrations of SOD (100 and 200 U/mL) on superoxide generation. Confluent HUVEC monolayers were pretreated for 1 h with SOD (100 and 200 U/ML) prior to 1 h of incubation with 5 ng/mL TNF. A representative set of images are shown. Bars represent mean values (mean ± SEM, n = 4 independent experiments). (#) P < 0.05, compared to untreated control. Bar graph having lighter color indicates that a particular treatment has a significant effect compared with TNF alone.