Role of IRW
and IQW on TNF induced NF-B activation: (A) confluent
HUVEC monolayers were treated with BAY 11-7085 (NF-B inhibitor) for
15 min before 4 h with 5 ng/mL TNF. ICAM-1 and VCAM-1 protein levels
are expressed as fold increase over the untreated control. Bars represent
mean values (mean ± SEM, n = 4 independent experiments).
Representative Western blots are shown below. (##) P < 0.01, compared to untreated control; (∗∗) P < 0.01, compared to TNF alone. (B, C) Confluent HUVEC
monolayers were pretreated for 20 h with 50 mol/L IRW and IQW prior
to 30 min of incubation with 5 ng/mL TNF. Cells were fixed, permeabilized,
and immunostained for p65 (B) and p50 (C). Representative sets of
images from six independent experiments are shown. Bar graph having
lighter color indicates that a particular treatment has a significant
effect compared with TNF alone.