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. 2013 Jan 15;61(9):2120–2129. doi: 10.1021/jf3046076

Figure 6.

Figure 6

Role of IRW and IQW on TNF induced NF-B activation: (A) confluent HUVEC monolayers were treated with BAY 11-7085 (NF-B inhibitor) for 15 min before 4 h with 5 ng/mL TNF. ICAM-1 and VCAM-1 protein levels are expressed as fold increase over the untreated control. Bars represent mean values (mean ± SEM, n = 4 independent experiments). Representative Western blots are shown below. (##) P < 0.01, compared to untreated control; (∗∗) P < 0.01, compared to TNF alone. (B, C) Confluent HUVEC monolayers were pretreated for 20 h with 50 mol/L IRW and IQW prior to 30 min of incubation with 5 ng/mL TNF. Cells were fixed, permeabilized, and immunostained for p65 (B) and p50 (C). Representative sets of images from six independent experiments are shown. Bar graph having lighter color indicates that a particular treatment has a significant effect compared with TNF alone.