Fig 1.

Schematic representation of iPCR assay for detection of Lyme disease biomarkers. Intact spirochete (A) or recombinant protein antigen coupled to magnetic beads (B) was used to capture B. burgdorferi-specific host-generated antibodies. A biotinylated DNA oligonucleotide reporter molecule coupled to a streptavidin-conjugated reporter antibody was amplified by qPCR for detection and quantification. (C) Anti-B. burgdorferi antibody coupled to magnetic beads was used for spirochete capture, with detection accomplished by qPCR amplification of the DNA oligonucleotide-coupled reporter antibody, similar to detection of host antibody.