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B. burgdorferi captured on magnetic beads provides a reagent for host antibody detection by iPCR. Phase-contrast microscopy and fluorescence microscopy at 510 nm (GFP [green fluorescent protein]) and ×400 magnification (400×) were used to determine capture of formalin-fixed B. burgdorferi expressing green fluorescent protein on beads coated with anti-B. burgdorferi (αBb) polyclonal antibodies (top) or uncoated beads (bottom).
