Table 1.
iPCR DNA oligonucleotide sequences used in this study
| Oligonucleotide no. | Oligonucleotide identification | Sequence (5′–3′)a |
|---|---|---|
| T1 | Template 1 (IgG coupled) | Biotin-AGCCTCAGACCAAGCCAGACAACTGCCTCGTGACGTTGCTGCCCCTACCAACGTACCCCTACGAGTCC |
| T1F | Template 1 forward | AGCCTCAGACCAAGCCAGAC |
| T1R | Template 1 reverse | GGACTCGTAGGGGTACGTTGG |
| T1P | Template 1 probe | FAM-ACTGCCTCGTGACGTTGCTGCCCCT-BHQ1 |
| T2 | Template 2 (IgM coupled) | Biotin-AGGAGGAGGGTCAAGTCACCAACGCTGCTCCAGGCCATCGTGCTGATCTGGACCCTGGATCGAGTGA |
| T2F | Template 2 forward | AGGAGGAGGGTCAAGTCACC |
| T2R | Template 2 reverse | TCACTCGATCCAGGGTCCAG |
| T2P | Template 2 probe | MAX-ACGCTGCTCCAGGCCATCGTGCTGA-BHQ1 |
| P1 | OspC partial HA forward | cgggatcccatatgTGTAATAATTCAGGGAAAGATGG |
| P2 | OspC HA reverse | acgcgtcgacTTAcgcataatccggcacatcatacggataAGGTTTTTTTGGACTTTCTGC |
| P3 | BmpA partial myc forward | cgggatcccatATGTGTAGTGGTAAAGGTAGTCTTG |
| P4 | BmpA myc reverse | acgcgtcgacTTAcagatcttcttcagaaataagtttttgttcAATAAATTCTTTAAGAAACTTCTCATAAC |
| P5 | C6 Bb forward | cgggatcccatATGAAGAAGGATGATCAGATTG |
| P6 | C6 Bb reverse | acgcgtcgacTTACTTCACAGCAAACTTTCCATC |
a
Lowercase letters indicate nontemplate sequence used for addition of terminal restriction sites and/or epitope tags. BHQ1, black hole quencher 1.