Skip to main content
. Author manuscript; available in PMC: 2013 Mar 8.
Published in final edited form as: J Immunol. 2009 Jul 10;183(3):1804–1812. doi: 10.4049/jimmunol.0804206

Figure 1. SLP-76 and BLNK are phosphorylated, in OCs, by integrin occupancy in a Syk-dependent manner.

Figure 1

(A) Wild-type BMMs, cultured with M-CSF and RANKL, with time, were lysed and the lysate immunoblotted for SLP-76 and BLNK. β3 integrin subunit (arrow) serves as a marker of OC differentiation. (B and C) Syk+/? and Syk−/− BMMs were cultured with M-CSF and RANKL for four days to generate pre-OCs. The cells were serum and cytokine starved, lifted with 0.5% EDTA and either re-plated on vitronectin-coated plates or maintained in suspension. Cleared cell lysates from adhered (A) or suspended (S) cells were immunoprecipitated for either SLP-76 (B) or BLNK (C) and the immunoprecipitates were immunoblotted for phosphotyrosine and SLP-76 or BLNK.