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. Author manuscript; available in PMC: 2013 Jun 20.
Published in final edited form as: Virology. 2012 Apr 21;428(1):70–75. doi: 10.1016/j.virol.2012.03.021

Fig. 2.

Fig. 2

Transcription from pG5MLT is p300 and acetyl-CoA dependent and is repressed by E1A 243R and E1A 1–80 but not by E1A 1–80 Δ4–25. Lane 1 shows transcription from naked DNA of both pG5MLT and pΔMLP. Lanes 2 to 7 show transcription from pG5MLT reconstituted chromatin and the naked DNA control pΔMLP. Components of chromatin transcription (Gal4-VP16, p300 and acetyl-CoA) as well as E1A polypeptides were added as indicated. Radio-labeled transcripts were visualized by phosphor-image analysis.