Skip to main content
. 2012 Oct 18;41(1):277–287. doi: 10.1093/nar/gks976

Figure 2.

Figure 2.

JQ1 stimulates predominantly Tat-dependent HIV transcription in both Jurkat and HeLa cells and ectopically expressed Tat is largely insufficient to overcome Brd4 inhibition of Tat-transactivation. (A) The Jurkat-based 1G5 cells containing an integrated HIV LTR-luciferase construct were nucleofected with the vector expressing Tat-HA (+) or nothing (−) and then treated with JQ1 or DMSO (−) as indicated. Whole cell extracts (WCE) were examined for the contained luciferase activities (top panel) and indicated proteins by western blotting (WB, bottom panel). (B and C) 1G5 cells were treated for 6 h (B) or 24 h (C) with the indicated concentrations of JQ1 or prostratin. WCE were examined for luciferase activities (top panels) and α-tubulin levels (bottom panels) as in A. (D) The HeLa-based NH1 cells expressing no Tat (left two columns) and NH2 cells stably expressing Tat-HA (right two columns), with both containing an integrated HIV LTR-luciferase construct, were treated with JQ1 or DMSO (−) and analysed as in A. (E) J-Lat A2 cells were treated as in A and analysed by FACS to determine the percentages of GFP(+) cells (top panel), by qRT-PCR for the GFP/GAPDH mRNA ratios (middle), and by WB for the indicated proteins in WCE (bottom). The error bars in all panels represent mean ± SD from three independent experiments.