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Observed overhangs at nascent termini of fragments resulting from RNA scission and/or fragmentation. R = 5′-fragment. R′ = 3′-fragment. Corresponding mass differences are shown in Table 2. Proposed mechanisms of formation of each overhang are shown in the Supplementary Data. *Monitored, but not a regularly observed cleavage product. An alternative nomenclature commonly used for the corresponding fragment ions in MS is shown where applicable (33–35).
