Figure 4.

Time-dependent cleavage of 10 µM Fl-RRE RNA (**) by 10 µM Fe–EDTA–Rev, 1 mM ascorbate and 1 mM H₂O₂ (see corresponding mass spectra in Figure 3). Cleavage fragments are organized by overhang type (A–F), location of the nascent overhang within the RNA sequence (5′ to 3′, left to right), and time-point within the incubation (0, 2, 5, 10, 60, 120 min, front to back). (A) 3′-hydroxyls (includes unreacted full-length Fl-RRE RNA), (B) 2′,3′-cyclic phosphates, (C) 3′-phosphates, (D) 3′-phosphoglycolates, (E) 5′-hydroxyls and (F) 5′-phosphates. From these data, apparent initial rates of formation were calculated for each product and are shown in the Supplementary Data, presented in the same layout as shown for cleavage of Fl-16S RNA by Cu–GGH/co-reactants in Figure 5C.