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. 2012 Oct 23;41(1):315–326. doi: 10.1093/nar/gks988

Figure 2.

Figure 2.

Effects of transcription inhibition and K+ import on the dissociation of RNAP from the nucleoid. (A) RNAP dissociation during osmotic response in rifampicin-treated cells. RNAP-venus protein fusion, nucleoid, overlay of RNAP (green) and nucleoid (red), overlay of nucleoid (white) and cell (black) before the treatment (LB), treated with rifampicin for 30 min only (LB + rif), and sequential treatments of rifampicin (30 min) and salt (20 min) (LB + rif + NaCl 20'). (B) K+ import is required for RNAP dissociation. RNAP-venus protein fusion, nucleoid, overlay of RNAP (green) and nucleoid (red), overlay of nucleoid (white) and cell (black) before (NaCl 0′) or after (NaCl 20′ and NaCl 60′) osmotic shock in the absence (M63-K) or presence (M63) of K+. (C) RNAP is not dissociated from the nucleoid in a K+ import defective strain (ΔkdpFAD ΔtrkAD) during osmotic stress response in LB. RNAP-venus protein fusion, nucleoid, overlay of RNAP (green) and nucleoid (red), overlay of nucleoid (white) and cell (black) before (LB) or after (NaCl 20′ and NaCl 60′) osmotic shock. The bar scale represents 2 µm.