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. 2012 Oct 23;41(1):315–326. doi: 10.1093/nar/gks988

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Published by Oxford University Press 2012.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.

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Figure 3. — The re-association of RNAP with the nucleoid is delayed in the double deletion mutant (ΔproPΔbetT) defective in the accumulation of organic osmoprotectant. RNAP-venus protein fusion, nucleoid, overlay of RNAP (green) and nucleoid (red), overlay of nucleoid (white) and cell (black) before (LB) or after (NaCl 20′ and NaCl 60′) osmotic shock. Note that the appearance of the ring of RNAP surrounding the hyper-condensed nucleoid is delayed compared with wild type (Figure 1B). The scale bar represents 2 µm.