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. 2012 Oct 23;41(1):315–326. doi: 10.1093/nar/gks988

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Published by Oxford University Press 2012.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.

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Figure 4. — Nucleoid-associated proteins behave similarly as RNAP during the osmotic stress response. Western blot using specific antibodies (β′, H-NS and IHF as indicated) against the total lysate (T), the soluble fraction (S) and the nucleoid fraction (N) from the cells before (LB) or after (NaCl 20′ and NaCl 60′) osmotic shock. After 20 min, increased fractions of RNAP, HNS and IHF are found in the cytoplasm compared with that before salt addition (LB); after 60 min, they become re-associated with the nucleoid. The nature of the other bands in the IHF blot is unknown.