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. 2012 Oct 23;41(1):315–326. doi: 10.1093/nar/gks988

Figure 5.

Figure 5.

Effects of inhibition of DNA supercoiling and dps deletion on the dissociation of RNAP and nucleoid structure during the initial osmotic stress response. (A) The inhibition of supercoiling by gyrase inhibitor coumermycin does not prevent RNAP dissociation nor the nucleoid to condense during the osmotic shock. RNAP-venus protein fusion, nucleoid, overlay of RNAP (green) and nucleoid (red), overlay of nucleoid (white) and cell (black) before (LB), after treatment with coumermycin for 30 min (Cr 30′), and after sequential treatments of coumermycin for 30 min first and then salt (Cr 30′ + NaCl 20′). Note that there is no ring of RNAP surrounding the hyper-condensed nucleoid. (B) The absence of Dps does not prevent RNAP dissociation, nor does it prevent the nucleoid to condense during the osmotic shock. RNAP-venus protein fusion, nucleoid, overlay of RNAP (green) and nucleoid (red), overlay of nucleoid (white) and cell (black) before (LB) or after (NaCl 20′) osmotic shock for the dps deletion mutant cells. The scale bar represents 2 µm.