Skip to main content
. 2012 Oct 18;41(1):264–276. doi: 10.1093/nar/gks958

Figure 2.

Figure 2.

CHX is a non-competitive inhibitor of both eEF3 and ATP. (A and D) Lineweaver–Burk plots of the disassembly reaction (mRNA release) inhibited by CHX at various concentrations of eEF3 (A) or ATP (D). (B and C) The slope (B) or Y intercept (C) of A was plotted against the concentrations of CHX. Ki and Ki’ of CHX were estimated to be 34 µM and 33 µM from B and C, respectively, where Ki and Ki’ are the dissociation constants for the enzyme–inhibitor (EI) complex or the enzyme–substrate–inhibitor (ESI) complex, respectively. (E and F) The slope (E) or Y intercept (F) of D was plotted against the concentrations of CHX. Ki and Ki’ of CHX were estimated to be 35 µM and 33 µM from E and F, respectively.