Figure 6.

Effects of CHX or PAR on ATP hydrolysis during the eEF3-catalysed disassembly of PoTC. The reaction mixture (15 µl) contained 0.1 µM PoTC, 0.5 µM eEF3, 50 µM [γ-³²P]ATP, and various concentrations of inhibitors in buffer 3/150. Incubation was carried out at 30°C for 1 min, and the hydrolysis of ATP was plotted against the concentrations of inhibitors. Vanadate [an inhibitor of ATPase (32)] is a positive control and indicates the partial inhibition of the ATPase of eEF3/ribosome.