Figure 4.

In situ hybridization showing expression pattern of hydra miRNAs. (A) In situ hybridization was performed using DIG-labelled locked nucleic acid probes for miRNAs hma-miR-2030, hma-miR-3005 and hma-miR-3010. lin-4-3p probe was used as a negative control, as it is not expressed in hydra. Inset shows expression pattern in ectoderm and endoderm at higher magnification. (B) Figure shows expression pattern of a perfect stem–loop structure gi_195009632_19624 seen after in situ hybridization.