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. 2012 Oct 30;41(1):340–354. doi: 10.1093/nar/gks974

Figure 8.

Figure 8.

DNA packaging in vitro of gp2 mutant forms. (A) DNA packaging in vitro was analyzed by a DNase protection assay as detailed under ‘Materials and Methods’ section in packaging reactions with the indicated purified gp2 constructs. Lane 1, control DNA; lane 6, control DNA treated with DNase. (B) Packaging efficiency of gp2 proteins carrying ATPase or nuclease mutations in packaging reactions performed in the presence of Mg2+ (upper panels) or Mn2+ (lower panels).