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. 2012 Oct 31;41(1):366–377. doi: 10.1093/nar/gks1018

Figure 3.

Figure 3.

Effect of σ70 mutations on the binding in the closed complex. (A) Binding isotherms of E. coli RNA polymerase (open circles) and E. coli RNA polymerase-σ70-Q437A (solid circle) with wild-type λ-PR promoter at 4°C. (B) ΔΔG values of Q437A and R436A mutants for wild-type galP1 and λ-PR promoters with respect to the wild-type RNA polymerase at 4°C. (C) ΔΔG values of Q437A and R436A mutant RNA polymerases for different galP1 mutant promoters from the corresponding wild-type template at 4°C.