Table 1.
Sequences and GC-contents of RNA substrates that were used in this study
| RNA | GC-content [%] | Sequences |
|---|---|---|
| 21R | 38.1 | 5’-AUGUGGAAAAUCUCUAGCAGU-3’ |
| 3’-UACACCUUUUAGAGAUCGUCA-5’ | ||
| JM1 | 4.8 | 5’-AAUUUAAUGUUUUAUUUAUUA-3’ |
| 3’-UUAAAUUACAAAAUAAAUAAU-5’ | ||
| J1h | 5’-UUAAUUUAAUGUUUUAUUUAUUAGGGUUUAUGGCUGUUCGCCAUUU–3’ | |
| JM2 | 14.3 | 5’-AAUUUAAUGUUUUAGUUACUA-3’ |
| 3’-UUAAAUUACAAAAUCAAUGAU-5’ | ||
| J2h | 5’-UUAAUUUAAUGUUUUAGUUACUAGGGUUUAUGGCUGUUCGCCAUUU–3’ | |
| JM3 | 23.8 | 5’-AAUUCAAUGUUUUAGUUACUG-3’ |
| 3’-UUAAGUUACAAAAUCAAUGAC-5’ | ||
| J3h | 5’-UUAAUUCAAUGUUUUAGUUACUGGGGUUUAUGGCUGUUCGCCAUUU–3’ | |
| JM4 | 33.3 | 5’-AACUCAAUGUCUUAGUUACUG-3’ |
| 3’-UUGAGUUACAGAAUCAAUGAC-5’ | ||
| J4h | 5’-UUAACUCAAUGUCUUAGUUACUGGGGUUUAUGGCUGUUCGCCAUUU–3’ | |
| JM6 | 52.4 | 5’-GACUCAGUGUCAGAGUCACUG-3’ |
| 3’-CUGAGUCACAGUCUCAGUGAC-5’ | ||
| J6h | 5’-UUGACUCAGUGUCAGAGUCACUGGGGUUUAUGGCUGUUCGCCAUUU–3’ |
The base-paired form of 21R is referred to as ds21R throughout the article while the single-strand (first line) is called 21R+. For the JM series, JM always refers to the double-strand and J (first line) and M (second line) are the single-strands. The RNAs termed J1h-J4h and J6h contain an additional 3′hairpin binding-platform (separated by GGGUUU from the base-pairing region) as well as a 5′-UU overhang. The nucleotides base-pairing with each other to form the stem of the hairpin are underlined.